Seven-enzyme in vitro cascade to (3R)-3-hydroxybutyryl-CoA?
Organic & Biomolecular Chemistry Pub Date: 2019-01-09 DOI: 10.1039/C8OB02858C
Abstract
Economical and environmentally-friendly routes to convert feedstock chemicals like acetate into valuable chiral products such as (R)-3-hydroxybutyrate are in demand. Here, seven enzymes (CoaA, CoaD, CoaE, ACS, BktB, PhaB, and GDH) are employed in a one-pot, in vitro, biocatalytic synthesis of (3R)-3-hydroxybutyryl-CoA, which was readily isolated. This platform generates not only chiral diketide building blocks but also desirable CoA derivatives.
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Journal Name：Organic & Biomolecular Chemistry
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CAS no.: 89640-58-4
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