Pyridoxal-thiosemicarbazide: its anion sensing ability and application in living cells imaging?
RSC Advances Pub Date: 2015-06-03 DOI: 10.1039/C5RA05059F
Abstract
A new anion selective chemosensor L was derived through a direct condensation reaction between pyridoxal and thiosemicarbazide. Sensor L showed selective recognition and sensing ability towards F? and AcO? anions through a naked-eye detectable color change from colorless to light yellow, appearance of a new charge transfer absorption band at 404 nm and significant “turn-on” fluorescence at 506 nm. The detection limit of L as a fluorescent ‘turn-on’ sensor for the analysis of F? and AcO? was estimated to be 0.10 μM. The anion sensing mechanisms of L was supported by 1H NMR and DFT results. Finally, the cytotoxicity effect of L and its ability to image intracellular F? ions in the living HeLa cells was investigated.
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Journal Name:RSC Advances
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CAS no.: 89640-58-4