A simple and high throughput parallel dual immunoaffinity liquid chromatography-mass spectrometry system for urine drug testing

Analytical MethodsANAL METHODS-UK Pub Date: 2018-01-22 DOI: 10.1039/C7AY02755A

Abstract

A simple and rapid method for direct quantitation of drugs in human urine samples was developed using a system composed of an automatic column switch and two home-made capillary immunoaffinity columns (CIACs, 100 μm × 15 cm). Before being injected by an autosampler into the system, the urine sample was filtered through a 0.22 μm membrane. The alternating column regeneration was achieved by using two identical CIACs. One column underwent analyte elution while the other column underwent column regeneration simultaneously. 6-Acetylmorphine (6AM) and oxycodone (OCOD) were studied as examples of opiates in urine. Linear calibration curves were obtained in the concentration ranges of 40–200 ng mL?1 and 50–400 ng mL?1, with a limit of quantitation of 22 ng mL?1 and 47 ng mL?1 for 6AM and OCOD, respectively. The results of this method exhibited good inter-day accuracy and precision. Furthermore, the present method was successfully applied to the determination of 6AM in urine samples of drug abusers.

Graphical abstract: A simple and high throughput parallel dual immunoaffinity liquid chromatography-mass spectrometry system for urine drug testing
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