Modified oligodeoxynucleotide primers for reverse-transcription of target RNAs that can discriminate among length variants at the 3′-terminus?
Organic & Biomolecular Chemistry Pub Date: 2013-10-02 DOI: 10.1039/C3OB41901K
Abstract
To discriminate among miRNA length variants, we synthesized conformationally restricted or unrestricted oligonucleotides containing a cyclohexyl phosphate residue. These oligonucleotides formed duplexes with length-matched complementary miRNAs more tightly than with length variants. The use of one of these modified oligodeoxynucleotides as a reverse transcription primer enabled a novel RT-PCR that can discriminate among miRNA length variants.
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Journal Name：Organic & Biomolecular Chemistry
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CAS no.: 89640-58-4
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