Kinetic evaluation of glucose 1-phosphate analogues with a thymidylyltransferase using a continuous coupled enzyme assay?
Organic & Biomolecular Chemistry Pub Date: 2014-10-30 DOI: 10.1039/C4OB02057J
Abstract
Cps2L, a thymidylytransferase, is the first enzyme in Streptococcus pneumoniaeL-rhamnose biosynthesis and an antibacterial target. We herein report the evaluation of six sugar phosphate analogues selected to further probe Cps2L substrate tolerance. A modified continuous spectrophotometric assay was employed for facile detection of pyrophosphate (PPi) released from nucleotidylyltransfase-catalysed condensation of sugar 1-phosphates and nucleoside triphosphates to produce sugar nucleotides. Additionally, experiments using waterLOGSY NMR spectroscopy were investigated as a complimentary method to evaluate binding affinity to Cps2L.
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Journal Name：Organic & Biomolecular Chemistry
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CAS no.: 89640-58-4
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