A novel chemiluminescence enhanced method for determination of putrescine in shrimp based on the luminol–[Ag(HIO6)2]5? reaction

Analytical Methods Pub Date: 2016-01-04 DOI: 10.1039/C5AY02596F

Abstract

A novel chemiluminescence (CL) method to determine putrescine was proposed based on the enhanced effect of putrescine on CL emission of the [Ag(HIO6)2]5?–luminol system. The enhanced degree of CL emission was proportional to the putrescine concentration. Under optimal conditions, there was a linear relationship between the increase in CL intensity and the putrescine concentration over a range of 4.0 × 10?8 to 4 × 10?6 g mL with a detection limit (3σ) of 1.76 × 10?8 g mL?1. The relative standard deviation (RSD) was 3.2% for 11 replicate determinations of 1.0 × 10?6 g mL?1 putrescine. The proposed CL reaction systems had potential capability for residue analysis of studied analytes in food samples. A possible mechanism of CL emission and enhanced effect was also suggested.

Graphical abstract: A novel chemiluminescence enhanced method for determination of putrescine in shrimp based on the luminol–[Ag(HIO6)2]5? reaction
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