Genetically encoded fluorescence screening probe for MgrA, a global regulator in Staphylococcus aureus?
RSC Advances Pub Date: 2015-10-08 DOI: 10.1039/C5RA11455A
Abstract
Herein, we construct a novel cell-based fluorescence response screening system based on a single insertion of circularly permuted yellow fluorescent protein (cpYFP) to reflect the protein conformational change caused by MgrA–small molecules interaction. And this screening platform was applied for screening of Chinese herb extracts with two extracts identified from 351.
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Journal Name：RSC Advances
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CAS no.: 89640-58-4
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