Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism?
Energy & Environmental Science Pub Date: 2018-09-25 DOI: 10.1039/C8EE01975D
Abstract
[FeFe]-Hydrogenases are hydrogen producing metalloenzymes with excellent catalytic capacities, highly relevant in the context of a future hydrogen economy. Here we demonstrate the synthetic activation of a heterologously expressed [FeFe]-hydrogenase in living cells of Synechocystis PCC 6803, a photoautotrophic microbial chassis with high potential for biotechnological energy applications. H2-Evolution assays clearly show that the non-native, semi-synthetic enzyme links to the native metabolism in living cells.
![Graphical abstract: Generation of a functional, semisynthetic [FeFe]-hydrogenase in a photosynthetic microorganism](http://scimg.chem960.com/usr/1/C8EE01975D.jpg)
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Journal Name：Energy & Environmental Science
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CAS no.: 89640-58-4
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