Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit?

Chemical Communications Pub Date: 2012-05-04 DOI: 10.1039/C2CC32038J

Abstract

A new aggregation-induced emission (AIE) compound 1,1-dimethyl-2,5-bis[4-(isothiocyanatemethyl)phenyl]-3,4-diphenylsilole (SITC) was synthesized and used to conjugate with aminoallyl-dUTP. The SITC窶電UTP can be incorporated enzymatically into DNA strands with the degree of labeling (DOL) up to the theoretic limit.

Graphical abstract: Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit
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