A high-throughput BIA-MPA method for the simultaneous determination of amiloride and furosemide

Analytical Methods Pub Date: 2016-10-14 DOI: 10.1039/C6AY02506D

Abstract

In this work, a simple and fast batch injection analysis system with multiple pulse amperometric detection (BIA-MPA) was developed for the simultaneous determination of amiloride (AMD) and furosemide (FMD). A sample plug (150 μL) was directly injected into the unmodified boron-doped diamond (BDD) electrode immersed in 0.1 mol L?1 borate buffer (pH = 10). Simple and fast sample pre-treatment steps were required (dissolution and dilution). The analytical characteristics of the proposed method include good stability (RSD < 1.1%; n = 20), low detection limits (0.13 and 0.94 mg L?1 for AMD and FMD, respectively), high throughput (72 injections per h) and minimal waste production (～150 μL by analysis). The results obtained with the BIA-MPA method were compared to those obtained by HPLC; similar results were obtained (at a 95% confidence level).

Graphical abstract: A high-throughput BIA-MPA method for the simultaneous determination of amiloride and furosemide
Recommended Literature