A high-affinity subtype-selective fluorescent probe for estrogen receptor β imaging in living cells?

Chemical Communications Pub Date: 2018-03-23 DOI: 10.1039/C8CC00483H

Abstract

Estrogen receptor β (ERβ) has recently been identified as a pharmaceutical target in hormone replacement therapy for breast cancers. However, the biological function of ERβ in disease progression remains unclear. A highly ERβ-selective fluorescent probe (FPNM) was discovered exhibiting nanomolar affinity for ERβ with an ERβ/ERα selectivity as high as 80, which allowed specific labeling of intracellular ERβ. Moreover, distinct ERβ dynamics in various cellular bio-settings such as prostate cancer (DU-145) or triple-negative breast cancer (MDA-MB-231) cells were directly observed for the first time viaFPNM staining.

Graphical abstract: A high-affinity subtype-selective fluorescent probe for estrogen receptor β imaging in living cells
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