Development of a matrix solid phase dispersion-high performance liquid chromatography-tandem mass spectrometric method for multiresidue analysis of 25 synthetic colorants in meat products

Analytical Methods Pub Date: 2013-11-18 DOI: 10.1039/C3AY41664J

Abstract

A novel high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method is developed for multiresidue analysis of twenty five synthetic colorants in meat matrices with matrix solid phase dispersion (MSPD). Good linearity was obtained with a correlation coefficient of 0.9935–0.9999. The quantitation limits (LOQs) for 12 acidic colorants were in the range from 0.89 μg kg?1 (acid orange 2) to 50.4 μg kg?1 (ponceau 4R) except for tartrazine, and the LOQs for 13 basic colorants were in the range from 0.52 μg kg?1 (Rhodamine B) to 4.19 μg kg?1 (disperse blue 7); among them the LOQ for 4 triphenylmethane colorants was in the range of 0.77–0.96 μg kg?1 which is better than the minimum required performance limit (MRPL) of 2 μg kg?1 for the sum of malachite green and leucomalachite green. The average recoveries for all analytes at three spiked levels were in the range of 75.4–123% except for Rhodamine B, (120–130%), with a RSD of <11%. The results showed that the content of 8 allowable food colorants and 17 banned synthetic colorants was lower than their LOQ level. This MSPD-HPLC-MS/MS method has the advantages of being rapid, sensitive, accurate, and high-throughput, and can be applied for multiresidue analysis of synthetic colorants in meat products.

Graphical abstract: Development of a matrix solid phase dispersion-high performance liquid chromatography-tandem mass spectrometric method for multiresidue analysis of 25 synthetic colorants in meat products
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