Direct determination of 3-chloro-1,2-propanediol esters in beef flavoring products by ultra-performance liquid chromatography tandem quadrupole mass spectrometry

RSC Advances Pub Date: 2016-11-29 DOI: 10.1039/C6RA23417H

Abstract

A sensitive ultra-performance liquid chromatography tandem quadrupole mass spectrometry (UPLC-TQ-MS) method coupled with amino solid-phase extraction was developed for the direct determination of 3-chloro-1,2-propanediol (3-MCPD) esters which were firstly detected in the natural beef flavoring products. The analyzed 3-MCPD esters in this paper contain three monoesters and six diesters. The validation data indicated that the proposed method provided good linearity, repeatability and sensitivity. The method showed a good linearity within the range of 40–200 μg kg?1 for monoesters and 20–100 μg kg?1 for diesters with the determination coefficients (R2) ranging from 0.9912 to 0.9993. The limits of detection (LODs) for monoesters and diesters of 3-MCPD were in the range of 0.04–5.0 μg kg?1 and 0.13–16.0 μg kg?1, respectively. The method accuracy was confirmed by higher sample recovery which ranged from 80.5 to 113.7%. The repeatability expressed as intra-day precision ranged from 0.8 to 9.9% and inter-day precision varied from 2.8 to 13.8%. The validated method was successfully applied for determination of 3-MCPD esters in beef flavoring products.

Graphical abstract: Direct determination of 3-chloro-1,2-propanediol esters in beef flavoring products by ultra-performance liquid chromatography tandem quadrupole mass spectrometry
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