Comparative suppression of NLRP3 inflammasome activation with LPS-induced inflammation by blueberry extracts (Vaccinium spp.)

RSC Advances Pub Date: 2017-06-01 DOI: 10.1039/C7RA02562A

Abstract

The aim of this study was to evaluate the anti-inflammation effects of blueberry extracts through NLRP3 inflammasome. The anti-inflammation activities of blueberry extracts were detected using lipopolysaccharide (LPS) activated the mononuclear macrophage (RAW264.7), and then detected the gene expression levels of NLRP3, Caspase-1, apoptosis-associated specked-like protein (ASC) and inflammatory factor including IL-1β, TNF-α, IL-6, and iNOS. Blueberry extracts could significantly inhibit the gene expression of IL-1β, TNF-α, IL-6, and iNOS, but the inhibitions were showed differently among the varieties of Blomidom (BD), North Country (NC) and JK-M7 (JK). The protein expressions of NLRP3 and Caspase-1 in the blueberry treated groups were significantly lower than the LPS treated group. Quercetin significantly inhibited LPS-induced ROS and inhibited the mRNA expression and protein levels of NLRP3 and Caspase-1 in RAW264.7 cells.

Graphical abstract: Comparative suppression of NLRP3 inflammasome activation with LPS-induced inflammation by blueberry extracts (Vaccinium spp.)
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