Acyl carnitines are a class of compounds involved in the transport and metabolism of fatty acids within cells. These molecules play a critical role in energy production by facilitating the transfer of fatty acyl groups from the cytosol to the mitochondria, where they can be oxidized through β-oxidation for ATP generation. Acyl carnitines are synthesized enzymatically from carnitine and a fatty acyl-CoA derivative, primarily in the liver and kidneys. They serve as important biomarkers for assessing lipid metabolism and have applications in research related to metabolic disorders, including diabetes and cardiovascular diseases. Additionally, these compounds are utilized in nutritional supplements due to their potential health benefits associated with improved lipid profile and enhanced muscle function.