2′-Hydroxychalcones are a class of natural compounds derived from flavonoids, which exhibit diverse biological activities. These compounds feature an enone double bond and a hydroxyl group at the 2′ position on the C ring. They are commonly found in various plants such as citrus fruits, tea, and medicinal herbs. Studies have shown that 2′-hydroxychalcones possess significant antioxidant properties, which can help protect cells from oxidative damage. Additionally, they exhibit potential anti-inflammatory, antimicrobial, and anticancer activities. The unique structural features of these compounds contribute to their wide range of biological effects, making them promising candidates for pharmaceutical development.