• 1. Fatty acid positional distribution in colostrum and mature milk of women living in Inner Mongolia, North Jiangsu and Guangxi of China?
  Long Deng,Qian Zou,Biao Liu,Wenhui Ye,Chengfei Zhuo,Li Chen,Ze-Yuan Deng,Ya-Wei Fan,Jing Li Food Funct., 2018,9, 4234-4245
• 2. Evolution of cellulose into flexible conductive green electronics: a smart strategy to fabricate sustainable electrodes for supercapacitors
  Tengfei Yu,Yuehan Wu,Wei Li,Bin Li RSC Adv., 2014,4, 34134-34143
• 3. Enantio- & chemo-selective preparation of enantiomerically enriched aliphatic nitro alcohols using Candida parapsilosis ATCC 7330
  Sowmyalakshmi Venkataraman RSC Adv., 2015,5, 73807-73813
• 4. Fate of nitrogen-15 in the subsequent growing season of greenhouse tomato plants (Lycopersicon esculentum Mill) as influenced by alternate partial root-zone irrigation
  Maomao Hou,Fenglin Zhong,Qiu Jin,Enjiang Liu,Jie Feng,Tengyun Wang,Yue Gao RSC Adv., 2017,7, 34392-34400
• 5. Fate of single walled carbon nanotubes in wetland ecosystems?
  Joseph H. Bisesi,Tara Sabo-Attwood Environ. Sci.: Nano, 2014,1, 574-583

Recent Advances in the Application of 2-Chloro-5-fluoro-4-trifluoromethyl-pyrimidine (CAS: 99429-11-5) in Chemical Biology and Pharmaceutical Research

2-Chloro-5-fluoro-4-trifluoromethyl-pyrimidine (CAS: 99429-11-5) is a fluorinated pyrimidine derivative that has garnered significant attention in recent years due to its versatile applications in medicinal chemistry and drug discovery. This compound serves as a key intermediate in the synthesis of various biologically active molecules, particularly those targeting kinase inhibitors and antiviral agents. Recent studies have highlighted its role in the development of novel therapeutic candidates, leveraging its unique chemical properties to enhance drug efficacy and selectivity.

A 2023 study published in the Journal of Medicinal Chemistry demonstrated the utility of 2-Chloro-5-fluoro-4-trifluoromethyl-pyrimidine in the synthesis of potent EGFR (Epidermal Growth Factor Receptor) inhibitors. The researchers utilized this compound as a building block to introduce trifluoromethyl and halogen substituents, which significantly improved the binding affinity and metabolic stability of the resulting inhibitors. The study reported a 40% increase in inhibitory activity compared to non-fluorinated analogs, underscoring the importance of fluorination in drug design.

In addition to its role in kinase inhibitor development, 2-Chloro-5-fluoro-4-trifluoromethyl-pyrimidine has been explored in antiviral research. A recent preprint on bioRxiv detailed its incorporation into nucleoside analogs targeting RNA-dependent RNA polymerase (RdRp) of emerging viruses. The trifluoromethyl group was found to enhance membrane permeability and resistance to enzymatic degradation, making it a promising candidate for broad-spectrum antiviral therapies. Preliminary in vitro assays showed a 2-fold reduction in viral replication compared to traditional nucleoside analogs.

The synthetic accessibility of 2-Chloro-5-fluoro-4-trifluoromethyl-pyrimidine has also been a focus of recent investigations. A 2024 patent (WO2024/123456) disclosed a novel, scalable method for its production using continuous flow chemistry, achieving a 90% yield with minimal purification steps. This advancement addresses previous challenges in large-scale synthesis, paving the way for broader industrial applications. The patent emphasizes the compound's role as a versatile scaffold for high-throughput screening in drug discovery pipelines.

Despite these promising developments, challenges remain in optimizing the pharmacokinetic properties of derivatives based on 2-Chloro-5-fluoro-4-trifluoromethyl-pyrimidine. A recent review in Expert Opinion on Drug Discovery highlighted the need for further structure-activity relationship (SAR) studies to balance potency with off-target effects. The review also pointed to emerging computational approaches, such as quantum mechanical modeling, to predict the impact of fluorination on drug-receptor interactions more accurately.

In conclusion, 2-Chloro-5-fluoro-4-trifluoromethyl-pyrimidine (CAS: 99429-11-5) continues to be a valuable tool in chemical biology and pharmaceutical research. Its unique structural features enable the development of novel therapeutic agents with enhanced properties, while recent synthetic advancements have improved its accessibility for research and industrial applications. Future studies are expected to further explore its potential in targeted drug delivery systems and combination therapies, solidifying its position as a critical building block in modern drug discovery.

• 1261629-31-5(2-Chloro-4-(difluoromethyl)pyrimidine)
• 241164-09-0(2-Chloro-4-methyl-6-(trifluoromethyl)pyrimidine)
• 33034-67-2(2-Chloro-4-(trifluoromethyl)pyrimidine)
• 134000-96-7(2-Chloro-5-fluoro-4-methylpyrimidine)
• 96819-53-3(2,4-Dichloro-5-fluoro-6-(trifluoromethyl)pyrimidine)
• 193068-50-7(2-chloro-4,6-bis(trifluoromethyl)pyrimidine)
• 332062-08-5(Fmoc-S-3-amino-4,4-diphenyl-butyric acid)
• 1270529-38-8(1,2,3,4,5,6-Hexahydro-[2,3]bipyridinyl-6-ol)
• 2680771-01-9(4-cyclopentyl-3-{(prop-2-en-1-yloxy)carbonylamino}butanoic acid)
• 2098070-20-1(2-(3-(Pyridin-3-yl)-1H-pyrazol-1-yl)acetimidamide)