Cas no 207738-08-7 (3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x))

3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x) is a chromogenic substrate widely used in biochemical and diagnostic applications, particularly in ELISA and immunohistochemistry. Its key advantages include high sensitivity and a low background signal, enabling precise detection of peroxidase activity. The dihydrochloride hydrate form enhances solubility in aqueous buffers, facilitating consistent performance in assays. The compound produces a blue color upon oxidation, which can be measured spectrophotometrically or further stabilized for quantitative analysis. Its stability under standard storage conditions ensures reliable results over time. This substrate is preferred for its rapid reaction kinetics and compatibility with a range of peroxidase-labeled conjugates.
3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x) structure
207738-08-7 structure
Product Name:3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x)
CAS No:207738-08-7
MF:C16H24Cl2N2O
MW:331.280562400818
MDL:MFCD00150104
CID:241913
PubChem ID:24900558
Update Time:2025-06-11

3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x) Chemical and Physical Properties

Names and Identifiers

    • 3,3 ,5,5 -Tetramethylbenzidine dihydrochloride hydrate
    • TMB-2HCl-2H2O
    • 3,3',5,5'-tetramethylbiphenyl-4,4'-diamine hydrochloride hydrate
    • [1,1'-Biphenyl]-4,4'-diamine,3,3',5,5'-tetramethyl-, hydrochloride, hydrate (1:2:?)
    • 3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE
    • TMB (hydrochloride hydrate)
    • TMB DIHYDROCHLORIDE
    • 3,3 inverted exclamation marka,5,5 inverted exclamation marka-Tetramethylbenzidine dihydrochloride hydrate
    • 3,3‘,5,5‘-Tetramethylbenzidine dihydrochloride hydrate
    • 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate
    • TMB 2HC1
    • 3,3',5,5'-Tetramethylbenzidine dihydrochloride
    • [1,1'-Biphenyl]-4,4'-diamine, 3,3',5,5'-tetramethyl-, dihydrochloride
    • (1,1'-Biphenyl)-4,4'-diamine, 3,3',5,5'-tetramethyl-, hydrochloride (1:2)
    • 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline dihydrochloride
    • (1,1'-Biphenyl)-4,4'-diamine, 3,3',5,5'-tetramethyl-, dihydrochloride
    • [1,1'-Biphenyl]-4,4'-diamine, 3,3',5,5
    • TMB dihydrochloride hydrate,98% (TMB*2HCl*2H2O)
    • 3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x)
    • MDL： MFCD00150104
    • Inchi： 1S/C16H20N2.2ClH.H2O/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;;/h5-8H,17-18H2,1-4H3;2*1H;1H2
    • InChI Key： KVCWTKDFVVSVSJ-UHFFFAOYSA-N
    • SMILES： Cl.Cl.O.NC1=C(C)C=C(C=C1C)C1C=C(C)C(=C(C)C=1)N

Computed Properties

  • Exact Mass: 312.11600
  • Isotope Atom Count: 0
  • Hydrogen Bond Donor Count: 4
  • Hydrogen Bond Acceptor Count: 2
  • Heavy Atom Count: 20
  • Rotatable Bond Count: 1
  • Complexity: 226
  • Covalently-Bonded Unit Count: 4
  • Defined Atom Stereocenter Count: 0
  • Undefined Atom Stereocenter Count : 0
  • Defined Bond Stereocenter Count: 0
  • Undefined Bond Stereocenter Count: 0
  • Surface Charge: 0
  • Tautomer Count: nothing
  • XLogP3: nothing
  • Topological Polar Surface Area: 52

Experimental Properties

  • Color/Form: Beige solid
  • Melting Point: >300°C
  • Solubility: Soluble in DMSO (~1.7 mg/ml), and 1:300 DMSO:PBS (pH 7.2) (0.03 mg/ml).
  • PSA: 52.04000
  • LogP: 6.51800
  • Solubility: Not available
  • Sensitiveness: Moisture & Light Sensitive

3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x) Security Information

  • Hazardous Material transportation number：NONH for all modes of transport
  • WGK Germany：3
  • Hazard Category Code： 22
  • Safety Instruction： S22; S24/25
  • TSCA：Yes
  • Storage Condition：2-8°C

3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x) Customs Data

  • HS CODE：2921590090
  • Customs Data：

    China Customs Code:

    2921590090

    Overview:

    2921590090. Other aromatic polyamines and derivatives and their salts. VAT:17.0%. Tax refund rate:13.0%. Regulatory conditions:nothing. MFN tariff:6.5%. general tariff:30.0%

    Declaration elements:

    Product Name, component content, use to

    Summary:

    2921590090. other aromatic polyamines and their derivatives; salts thereof. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:30.0%

3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x) Pricemore >>

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3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x) Suppliers

Tiancheng Chemical (Jiangsu) Co., Ltd
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(CAS:207738-08-7)3,3`,5,5`-Tetramethylbenzidinedihydrochloridehydrate
Order Number:LE9655
Stock Status:in Stock
Quantity:25KG,200KG,1000KG
Purity:99%
Pricing Information Last Updated:Friday, 20 June 2025 12:01
Price ($):discuss personally

3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x) Related Literature

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Additional information on 3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x)

Introduction to 3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x) and Its Significance in Modern Chemical Biology

3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x), identified by the CAS number 207738-08-7, is a compound of considerable interest in the field of chemical biology and pharmaceutical research. This compound, a derivative of benzidine, has garnered attention due to its unique chemical properties and its potential applications in various biochemical assays and diagnostic tools. The precise molecular structure of 3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x), characterized by its tetramethyl substitution pattern and the presence of hydrochloride and hydrate ions, contributes to its stability and reactivity under specific conditions, making it a valuable reagent in laboratory settings.

The significance of 3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x) lies in its role as a key intermediate in the synthesis of various bioactive molecules. Its ability to participate in redox reactions makes it particularly useful in enzyme-linked immunosorbent assays (ELISAs) and other immunochemical detection methods. Recent advancements in the field have highlighted its application in the development of novel diagnostic kits for the detection of certain biomarkers associated with neurological disorders. The compound's stability under controlled conditions ensures consistent performance in experimental protocols, which is crucial for obtaining reliable and reproducible results.

In the realm of pharmaceutical research, 3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x) has been explored for its potential as a precursor in the synthesis of therapeutic agents. Its structural motifs are reminiscent of more complex molecules that have shown promise in treating conditions such as cancer and autoimmune diseases. Researchers have leveraged its reactivity to develop novel compounds that exhibit enhanced binding affinity to target proteins. For instance, studies have demonstrated that derivatives of 3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x) can be engineered to modulate enzyme activity, offering a new avenue for drug development.

The CAS number 207738-08-7 provides a unique identifier for this compound, facilitating its recognition and use in scientific literature and databases. This standardized nomenclature ensures that researchers worldwide can accurately reference and utilize 3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x) in their studies without ambiguity. The compound's molecular formula and structural details are well-documented, allowing for precise synthetic routes to be developed. These details are critical for ensuring the purity and efficacy of the final product.

Recent research has also highlighted the role of 3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x) in environmental monitoring applications. Its ability to form complexes with metal ions has been exploited for the detection of heavy metals in water samples. This application is particularly relevant given the increasing concern over water pollution and the need for reliable methods to monitor environmental quality. The compound's sensitivity and selectivity make it an attractive candidate for use in field-based analytical techniques.

The synthesis of 3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x) involves a series of carefully controlled chemical reactions that highlight the expertise required in organic synthesis. The process typically begins with the condensation of appropriate precursors under inert conditions to ensure optimal yield and purity. The subsequent steps involve purification techniques such as recrystallization or column chromatography to isolate the desired product. These synthetic methodologies are critical for producing high-quality material that meets the stringent requirements of biochemical applications.

The hydrate form of 3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x) adds an additional layer of complexity to its handling and storage requirements. The presence of water molecules within the crystal lattice necessitates careful control over humidity levels during storage to prevent degradation. This aspect underscores the importance of proper storage conditions for maintaining the integrity of sensitive chemical compounds like this one.

In conclusion, 3, 3, 5, 5, '-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x) is a multifaceted compound with significant applications across various domains of chemical biology and pharmaceutical research. Its unique properties make it indispensable in diagnostic assays, environmental monitoring, and drug development efforts. As research continues to uncover new applications for this compound, it will undoubtedly play an even greater role in advancing scientific knowledge and technological innovation.

207738-08-7 (3,3',5,5'-Tetramethylbenzidine Dihydrochloride Hydrate (1:2:x)) Related Products

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Tiancheng Chemical (Jiangsu) Co., Ltd
(CAS:207738-08-7)3,3`,5,5`-Tetramethylbenzidinedihydrochloridehydrate
LE9655
Purity:99%
Quantity:25KG,200KG,1000KG
Price ($):Inquiry
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