Cas no 19333-63-2 (TNBT Diformazan)
TNBT Diformazan (Tetranitroblue tetrazolium diformazan) is a water-insoluble formazan dye commonly employed as a chromogenic substrate in histochemical and biochemical assays. Its key advantage lies in its high sensitivity for detecting dehydrogenase and reductase activity, particularly in NAD(P)H-dependent enzymatic reactions. The reduction of TNBT yields an intensely colored, stable diformazan precipitate, facilitating clear visualization under microscopy. Its nitro groups enhance electron affinity, improving reactivity with reducing equivalents. TNBT Diformazan is valued for its low background interference and compatibility with various embedding media, making it suitable for tissue staining and viability assays. The compound’s stability under standard laboratory conditions ensures reproducible results in quantitative and qualitative analyses.
TNBT Diformazan structure
Product Name:TNBT Diformazan
CAS No:19333-63-2
MF:C40H30N12O10
MW:838.740606784821
MDL:MFCD00059831
CID:123490
PubChem ID:87563385
Update Time:2025-10-16
TNBT Diformazan Chemical and Physical Properties
Names and Identifiers
-
- Diazene,1,1'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[2-[(4-nitrophenyl)[(4-nitrophenyl)hydrazono]methyl]-(9CI)
- TNBT Diformazan
- Diazene,1,1'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[2-[(4-nitrophenyl)[(4-nitrophenyl)hy...
- N-[2-methoxy-4-[3-methoxy-4-[[(Z)-N-(4-nitroanilino)-C-(4-nitrophenyl)carbonimidoyl]diazenyl]phenyl]phenyl]imino-4-nitro-N'-(4-nitroanilino)benzenecarboximidamide
- 1,1'-(3,3'-Dimethoxy-4,4'-diphenylene)bis-3,5-di(p-nitrophenyl)diformazan
- 3,5,3',5'-Tetrakis-(4-nitro-phenyl)-1,1'-(3,3'-dimethoxy-biphenyl-4,4'-diyl)-di-formazan
- 4,4'-Bis-[3,N'''-(4-nitro-phenyl)-formazano]-3,3'-dimethoxy-biphenyl
- B0282
- Tetranitro Blue Tetrazolium Diformazan
- Diformazan
- TetranitroBT diformazan
- 1,1'-(3,3'-Dimethoxy-1,1'-biphenyl-4,4'-diyl)bis[3,5-bis(4-nitrophenyl)formazan]
- 1,1'-(3,3'-Dimethoxy-4,4'-diphenylene)bis-3,5-di(p-nitrophenyl)diformazan Tetranitro Blue Tetrazolium Diformazan
- tnbt-diformazan
- (E,E)-1,1'-(3,3'-Dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis({(Z)-(4-nitrophenyl)[2-(4-nitrophenyl)hydrazinylidene]methyl}diazene)
- 19333-63-2
- MFCD00059831
- TNBT Diformazan, AR
- DTXSID80659858
-
- MDL： MFCD00059831
- Inchi： 1S/C40H30N12O10/c1-61-37-23-27(7-21-35(37)43-47-39(25-3-13-31(14-4-25)49(53)54)45-41-29-9-17-33(18-10-29)51(57)58)28-8-22-36(38(24-28)62-2)44-48-40(26-5-15-32(16-6-26)50(55)56)46-42-30-11-19-34(20-12-30)52(59)60/h3-24,41-42H,1-2H3/b45-39-,46-40-,47-43+,48-44+
- InChI Key： SDKSRZQMARNUEW-FOJIDQNJSA-N
- SMILES： O(C)C1=C(C=CC(=C1)C1C=CC(=C(C=1)OC)/N=N/C(/C1C=CC(=CC=1)[N+](=O)[O-])=N\NC1C=CC(=CC=1)[N+](=O)[O-])/N=N/C(/C1C=CC(=CC=1)[N+](=O)[O-])=N\NC1C=CC(=CC=1)[N+](=O)[O-]
Computed Properties
- Exact Mass: 838.22100
- Monoisotopic Mass: 838.22078520g/mol
- Isotope Atom Count: 0
- Hydrogen Bond Donor Count: 2
- Hydrogen Bond Acceptor Count: 16
- Heavy Atom Count: 62
- Rotatable Bond Count: 13
- Complexity: 1500
- Covalently-Bonded Unit Count: 1
- Defined Atom Stereocenter Count: 0
- Undefined Atom Stereocenter Count : 0
- Defined Bond Stereocenter Count: 2
- Undefined Bond Stereocenter Count: 0
- Surface Charge: 0
- Tautomer Count: nothing
- XLogP3: 10.4
- Topological Polar Surface Area: 300
Experimental Properties
- Color/Form: Uncertain
- Density: 1.5±0.1 g/cm3
- Boiling Point: 980.9±75.0 °C at 760 mmHg
- Flash Point: 547.0±37.1 °C
- PSA: 299.96000
- LogP: 12.35760
- Solubility: Uncertain
- Vapor Pressure: 0.0±0.3 mmHg at 25°C
TNBT Diformazan Security Information
-
Symbol:
- Prompt：warning
- Signal Word：warning
- Hazard Statement： H228
- Warning Statement： P210-P240-P241-P280
- Hazardous Material transportation number：1325
- Safety Instruction： H303+H313+H333
- HazardClass：4.1
- PackingGroup：III
- Storage Condition：storage at -4℃ (1-2weeks), longer storage period at -20℃ (1-2years)
TNBT Diformazan Pricemore >>
| Related Categories | No. | Product Name | Cas No. | Purity | Specification | Price | update time | Inquiry |
|---|---|---|---|---|---|---|---|---|
| SHANG HAI XIAN DING Biotechnology Co., Ltd. | B0282-1g |
TNBT Diformazan |
19333-63-2 | 1g |
￥420.0 | 2022-05-30 | ||
| SHANG HAI XIAN DING Biotechnology Co., Ltd. | B0282-100mg |
TNBT Diformazan |
19333-63-2 | 100mg |
￥100.0 | 2022-05-30 | ||
| TRC | T201805-100mg |
TNBT Diformazan |
19333-63-2 | 100mg |
$190.00 | 2023-05-17 | ||
| TRC | T201805-250mg |
TNBT Diformazan |
19333-63-2 | 250mg |
$345.00 | 2023-05-17 | ||
| TRC | T201805-500mg |
TNBT Diformazan |
19333-63-2 | 500mg |
$511.00 | 2023-05-17 | ||
| SHANG HAI MAI KE LIN SHENG HUA Technology Co., Ltd. | T871520-100mg |
TNBT Diformazan |
19333-63-2 | AR | 100mg |
￥275.40 | 2022-08-31 | |
| SHENG KE LU SI SHENG WU JI SHU | sc-485599-100 mg |
TNBT Diformazan, |
19333-63-2 | 100MG |
￥842.00 | 2023-07-11 | ||
| SHENG KE LU SI SHENG WU JI SHU | sc-485599-100mg |
TNBT Diformazan, |
19333-63-2 | 100mg |
￥842.00 | 2023-09-05 | ||
| 1PlusChem | 1P003VAR-1g |
TNBT DIFORMAZAN |
19333-63-2 | 1g |
$260.00 | 2025-02-20 | ||
| Aaron | AR003VJ3-250mg |
TNBT Diformazan |
19333-63-2 | 250mg |
$119.00 | 2025-01-22 |
TNBT Diformazan Related Literature
-
1. Fatty acid eutectic mixtures and derivatives from non-edible animal fat as phase change materials?Pau Gallart-Sirvent,Marc Martín,Gemma Villorbina,Mercè Balcells,Aran Solé,Luisa F. Cabeza,Ramon Canela-Garayoa RSC Adv., 2017,7, 24133-24139
-
Jason Y. C. Lim,Yong Yu,Guorui Jin,Kai Li,Yi Lu,Jianping Xie Nanoscale Adv., 2020,2, 3921-3932
-
Thi Thu Tram Nguyen,Thanh Binh Nguyen Org. Biomol. Chem., 2021,19, 6015-6020
-
Hongxia Li,Aikifa Raza,Qiaoyu Ge,Jin-You Lu,TieJun Zhang Soft Matter, 2020,16, 6841-6849
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