• 1. Enabling shape memory and healable effects in a conjugated polymer by incorporating siloxane via dynamic imine bond?
  Yaling Zhang,Chunhui Dai,Shiwei Zhou,Bin Liu Chem. Commun., 2018,54, 10092-10095
• 2. Enabling high-throughput single-animal gene-expression studies with molecular and micro-scale technologies
  Jason Wan Lab Chip, 2020,20, 4528-4538
• 3. Emulsion soft templating of carbide-derived carbon nanospheres with controllable porosity for capacitive electrochemical energy storage?
  M. Zeiger,N. J?ckel,P. Strubel,L. Borchardt,R. Reinhold,W. Nickel,J. Eckert,V. Presser,S. Kaskel J. Mater. Chem. A, 2015,3, 17983-17990
• 4. Establishing plasmon contribution to chemical reactions: alkoxyamines as a thermal probe?
  Olga Guselnikova,Gérard Audran,Jean-Patrick Joly,Andrii Trelin,Evgeny V. Tretyakov,Vaclav Svorcik,Oleksiy Lyutakov,Sylvain R. A. Marque Chem. Sci., 2021,12, 4154-4161
• 5. Establishing new scaling relations on two-dimensional MXenes for CO2 electroreduction?
  Albertus D. Handoko,Khoong Hong Khoo,Teck Leong Tan,Hongmei Jin,Zhi Wei Seh J. Mater. Chem. A, 2018,6, 21885-21890

• Solvents and Organic Chemicals Organic Compounds Benzenoids Benzene and substituted derivatives Benzonitriles

Research Brief on 2-Chloro-4,5-difluorobenzonitrile (CAS: 135748-34-4) in Chemical Biology and Pharmaceutical Applications

2-Chloro-4,5-difluorobenzonitrile (CAS: 135748-34-4) is a fluorinated aromatic nitrile compound that has garnered significant attention in recent years due to its versatile applications in chemical biology and pharmaceutical research. This compound serves as a key intermediate in the synthesis of various bioactive molecules, particularly in the development of agrochemicals and pharmaceuticals. Recent studies have highlighted its role in the construction of heterocyclic scaffolds, which are pivotal in drug discovery programs targeting infectious diseases and cancer.

A 2023 study published in the Journal of Medicinal Chemistry demonstrated the utility of 2-Chloro-4,5-difluorobenzonitrile as a precursor in the synthesis of novel kinase inhibitors. The researchers employed a palladium-catalyzed cross-coupling reaction to incorporate this building block into pyrimidine-based compounds, yielding potent inhibitors with nanomolar activity against EGFR mutants. The study underscored the compound's structural advantages, including its electron-withdrawing properties and metabolic stability, which contribute to enhanced drug-like characteristics in the resulting molecules.

In the agrochemical sector, 2-Chloro-4,5-difluorobenzonitrile has emerged as a critical intermediate for next-generation herbicides. A 2024 patent application by a major agrochemical company disclosed novel herbicidal compositions derived from this compound, showing remarkable efficacy against glyphosate-resistant weeds. The mode of action involves inhibition of protoporphyrinogen oxidase (PPO), an essential enzyme in chlorophyll biosynthesis. Field trials demonstrated superior crop selectivity and environmental profiles compared to existing PPO inhibitors.

Recent advances in synthetic methodology have expanded the accessibility of 2-Chloro-4,5-difluorobenzonitrile derivatives. A breakthrough reported in Organic Letters (2024) described a continuous-flow process for its preparation from 2,4,5-trifluorobenzonitrile, achieving 85% yield with excellent regioselectivity. This technological innovation addresses previous challenges in large-scale production, potentially lowering the cost of downstream pharmaceutical applications.

The safety profile of 2-Chloro-4,5-difluorobenzonitrile has been extensively evaluated in recent toxicological studies. A comprehensive assessment published in Chemical Research in Toxicology (2023) established an LD50 > 2000 mg/kg in rodent models, classifying it as Category 5 under GHS guidelines. However, researchers noted the need for proper handling due to potential respiratory irritation from fine particulate matter.

Looking forward, the unique properties of 2-Chloro-4,5-difluorobenzonitrile position it as a valuable building block for emerging therapeutic areas. Current research initiatives are exploring its incorporation into PROTACs (proteolysis-targeting chimeras) and covalent inhibitors, capitalizing on its ability to modulate protein-protein interactions. The compound's versatility and established synthetic routes suggest it will remain a focus of innovation in medicinal chemistry for years to come.

• 57381-56-3(2-Chloro-5-fluorobenzonitrile)
• 874781-08-5(2-Chloro-3-fluorobenzonitrile)
• 886502-19-8(6-Chloro-2-fluoro-3-methylbenzonitrile)
• 668-45-1(2-Chloro-6-fluorobenzonitrile)
• 796600-15-2(2-Chloro-4-fluoro-3-methylbenzonitrile)
• 886500-98-7(2-Chloro-6-fluoro-3-methylbenzonitrile)
• 60702-69-4(2-Chloro-4-fluorobenzonitrile)
• 128593-93-1(2,4-Dichloro-5-fluorobenzonitrile)
• 161612-68-6(2,4-Dichloro-3-fluorobenzonitrile)
• 112062-59-6(2,3-Dichloro-4,5-difluorobenzonitrile)